Ollie Watkins has been sent home from England's camp with an injury.
The Aston Villa forward scored in England's 3-0 win over Wales on Thursday evening, but sustained a knock against them and has not recovered.
He will miss their World Cup qualifier against Latvia on Tuesday, where the Three Lions could secure their place at the 2026 World Cup.
In a statement on Monday morning, the FA said: 'Ollie Watkins has been ruled out of England's 2026 FIFA World Cup qualifier in Latvia.
'The Aston Villa striker has not recovered from the knock sustained against Wales at Wembley on Thursday evening and has returned to his club. A squad of 22 will travel to Riga.'
Watkins' absence creates a selection headache for Thomas Tuchel after Harry Kane missed the victory over Wales through injury.
Aston Villa star Ollie Watkins has been forced to withdraw from England's squad due to injury
Kane suffered a foot injury in Bayern Munich's final game before the international break, although he is expected to be fit for the Latvia clash.
Speaking ahead of the Wales game, Tuchel had said: 'Harry will miss the game. He got a kick in his last match with Bayern Munich.
'It was too painful to kick a ball, it was too risky that he gets another kick and will just like be in an up and down situation pain-wise, so we gave him the chance that everything calms down.
'We are convinced that he will be ready for the match against Latvia.'